PLGA/피브린 지지체가 늑연골 세포의 부착과 성장에 미치는 영향

송정은; 이유정; 이윤미; 조선아; 장지은; 이동원; 강길선; Jeong Eun Song; Yujung Lee; Yun Me Lee; Sun Ah Cho; Ji Eun Jang; Dongwon Lee; Gilson Khang

Polymer(Korea), Vol.37, No.2, 141-147, March, 2013

Export Citation

PLGA/피브린 지지체가 늑연골 세포의 부착과 성장에 미치는 영향

Effects of PLGA/Fibrin Scaffolds on Attachment and Proliferation of Costal Cartilage Cells

송정은 , 이유정, 이윤미, 조선아, 장지은, 이동원 , 강길선 ^†

전북대학교 고분자나노공학과, BIN 융합공학과, 유기신물질공학과

Jeong Eun Song , Yujung Lee, Yun Me Lee, Sun Ah Cho, Ji Eun Jang, Dongwon Lee , and Gilson Khang^†

Dept. of BIN Fusion Tech & Dept. of PolymerNano Sci Tech, Dept. of Advanced Organic Materials Engineering, Chonbuk National University, 567 Baekje-daero, Jeonju 561-756, Korea

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초록

Poly(lactide-co-glycolic acid)(PLGA)는 좋은 기계적 성질과 생분해성으로 약물전달시스템 또는 조직공학적으로 널리 이용되고 있으나 낮은 세포 부착률을 가지고 있어 피브린을 첨가하여 이를 보완하고자 하였다. 본 연구에서 사용된 지지체는 트롬빈과 피브리노겐, 그리고 세포을 혼합시킨 후 PLGA 지지체 위에 도포시켜 제조하였다. 세포의 부착 및 증식률을 측정하고자 PLGA/피브린 지지체에 늑연골 세포를 파종 후 1, 3일 및 7일 후 SEM과 MTT 분석을 통하여 측정하였으며, 세포외기질 형성에 미치는 피브린의 영향을 확인하고자 세포를 파종 후 누드마우스에 이식하여 GAG 및 콜라겐 합성의 효과를 확인하였다. 따라서 본 연구에서는 피브린이 혼합된 PLGA 지지체가 생체 내·외 환경에서 세포의 부착 및 증식에 미치는 영향을 확인하고자 연구를 진행하였다. 그 결과, PLGA/피브린 지지체가 기존의 PLGA 지지체와 비교하여 탁월한 세포 성장률을 나타내는 것으로 확인하였다.

Poly(lactide-co-glycolic acid) (PLGA) has been widely used in the drug delivery and tissue engineering applications because of its good mechanical strength and biodegradation profile. However, cell attachment to the scaffold is low compared with that on fibrin although cells can be attached to the polymer surface. In this study, PLGA scaffolds were soaked in cells-fibrin suspension and polymerized with dropping fibrinogen-thrombin solution. Cellular proliferation activity was observed in PLGA/fibrin-seeded costal cartilage cells (CC) on 1, 3, and 7 days using the MTT assay and SEM. The effects of fibrin on the extracellular matrix (ECM) formation were evaluated using CC cell-seeded PLGA/fibrin scaffolds. The PLGA/fibrin scaffolds elicited more production of glycosaminoglycan (GAG) and collagen than the PLGA scaffold. In this study, fibrin incorporated PLGA scaffolds were prepared to evaluate the effects of fibrin on the cell attachment and proliferation in vitro and in vivo. In this result, we confirmed that proliferation of cells in PLGA/fibrin scaffolds were better than in PLGA scaffolds. The PLGA/fibrin scaffolds provide suitable environment for growth and proliferation of costal cartilage cells.

Keywords:fibrin;PLGA;scaffold;costal cartilage;extracellular matrix.

[References]

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Ko YK, Kim SH, Jeong JS, Park JS, Lim JY, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(6), 505 (2007)
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Song Y, Yoo H, Eum S, Kim OY, Yoo SC, Kim HE, Lee D, Khang G, Polym.(Korea), 35(3), 189 (2011)
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Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Bio. Mater. Res., 71B, 380 (2004)
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Solchaga LA, Temenoff JS, Gao J, Mikos AG, Caplan AI, Goldberg VM, Osteoarthr. Cartil., 13, 297 (2005)

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[1] Hardingham T, Tew S, Murdoch A, Arthritis. Res., 3, S63 (2002)

[2] Lee J, Lee E, Kim HY, Son Y, Biotechnol. Appl.Biochem., 48, 149 (2004)

[3] Kim SJ, Hong HH, Kim SH, Kim HL, Kim SH, Khang G, Polym.(Korea), 34(1), 63 (2010)

[4] Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)

[5] Shin E, Yoo SC, Song BJ, Kim YK, Park HJ, Seong KY, Song YS, Lee D, Khang G, Int. J. Tissue. Reg., 1, 28 (2010)

[6] Khang G, Lee SJ, Kim MS, “Scaffold; tissue engineering”, in Weber's Biomedical Engineering Handbook, 2nd Ed., Marcel Dekker, New York, 1, 336 (2006)

[7] Jo EH, Lee GY, Cho SJ, Yoo H, Kim OY, Seong K, Kang YS, Lee D, Khang G, Biomed. Eng. Appl. Basis Commun., 23, 119 (2011)

[8] Lee OJ, Lee JM, Jin HJ, Park CH, Inter. J. Tissue Regen., 1, 68 (2010)

[9] Park HN, Lee JB, Kwon IK, Inter. J. Tissue Regen., 1, 10 (2010)

[10] Klose D, Delplace C, Siepmann J, Int. J. Pharm., 404, 75 (2011)

[11] Fu K, Pack DW, Klibanov AM, Langer R, Pharm. Res., 17, 100 (2000)

[12] Sha'ban M, Yoon SJ, Ko YK, Ha HJ, Kim SH, So JW, Idrus RBH, Khang G, J. Biomater. Sci. Polym. Edn., 19, 1219 (2008)

[13] Buyuklu F, Hizal E, Yilmaz Z, Sahin FI, Cakmak O, J.Craniomaxillofac. Surg., 39, 225 (2011)

[14] Lau AG, Kindig MW, Kent RW, Acta Biomaterialia., 7, 1202 (2011)

[15] Liu Y, Shu XZ, Gray SD, Prestwich GD, J. Biomed.Mater. Res., 68A, 142 (2004)

[16] Yoo HS, Lee EA, Yoon JJ, Park TG, Biomaterials., 26, 1925 (2005)

[17] Sung HJ, Meredith C, Johnson C, Galis ZS, Biomaterials., 25, 5742 (2004)

[18] Huang W, Shi X, Ren L, Du C, Wang Y, Biomaterials., 31, 4285 (2010)

[19] Urist MR, Science., 150, 893 (1965)

[20] Ko YK, Kim SH, Jeong JS, Park JS, Lim JY, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(6), 505 (2007)

[21] Lu H, Hoshiba T, Kawazoe N, Chen G, Biomaterials., 32, 2499 (2011)

[22] Song Y, Yoo H, Eum S, Kim OY, Yoo SC, Kim HE, Lee D, Khang G, Polym.(Korea), 35(3), 189 (2011)

[23] Nogami H, Urist MR, J. Cell. Biol., 62, 519 (1974)

[24] Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Bio. Mater. Res., 71B, 380 (2004)

[25] Gutierrez BMC, Carvajal ZYG, Jobbagy M, Rubio F, Yuste L, Rojo F, Ferrer ML, Monte F, Adv. Funct. Mater., 17, 3513 (2007)

[26] Oh SH, Kang SG, Lee JH, J. Mater. Sci. Mater. Med., 17, 137 (2006)

[27] Murphy WL, Peters MC, Kohn DH, Mooney DJ, Biomaterials., 21, 2521 (2000)

[28] Solchaga LA, Temenoff JS, Gao J, Mikos AG, Caplan AI, Goldberg VM, Osteoarthr. Cartil., 13, 297 (2005)