| 학회 | 한국화학공학회 |
| 학술대회 | 2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 | 17권 2호, p.1874 |
| 발표분야 | 재료 |
| 제목 | Colorimetric detection of K-ras mutations using aggregation of PNA modified AuNPs by electrostaticattraction |
| 초록 | We report a colorimetric detection of K-ras mutations using selective aggregation of PNA modified AuNPs by electrostatic attraction. First, AuNPs are modified each PNA probes that are complementary to wild and mutant type target sequences, which made surface charge of AuNPs be neutral due to neutrally charged PNA. When the complementary targetDNA are added to AuNPs suspension, AuNPs represent a lower negative zeta potential than when the mismated targetDNA are added. In the second step, the hybridized AuNPs suspension add to positively charged AuNPs, which causes electrostatic attraction between the negatively charged PNA modified AuNPs and positively charged AuNPs. This result in substatial aggregation AuNPs, followed by color change in suspension from red to blue. However AuNPs did not exihibit significant aggregation, and remain suspension color contaning mismatched target DNA. This result suggest that detection of K-ras mutations using this method makes clinical benefit be predicted as well as patient selection easy. Aknowledgement :This work was supported by the Korea Research Foundation Grant (KRF-2009-0072530) |
| 저자 | 김아름, 이강택, 박승우 |
| 소속 | 연세대 |
| 키워드 | K-ras mutations; dna detection; gold nanoparticle; electrostatic interaction |
| 원문파일 | 초록 보기 |
