| 초록 |
Systems metabolic engineering approaches were applied to elucidate crucial factors affecting the biosynthesis of L-threonine in E.coli. We first developed the model strain, which can produce L-threonine by introducing targeted genetic modifications into prototrophic E.coli W3110. The L-threonine-producing strain was constructed by releasing regulatory mechanisms, such as feedback inhibition and attenuation. This strain also carries point mutations in ilvA, and lysA, metA and tdh gene. Next, we carried out comparative transcriptome analysis to identify the effect of L-threonine biosynthesis on the cellular physiology, and also undertook in silico simulation approaches especially to elucidate knock-out and amplification gene targets for the enhancement of L-threonine productivity. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10309020000-03B5002-00000). Further supports by LG Chem Chair Professorship, Microsoft and IBM SUR program are appreciated.] |
