| 초록 |
Direct lineage conversion of somatic cell into another functional cell type constitutes an attractive approach for research and clinical use. For the ultimate application of transdifferentiation for cell replacement therapy or in vivo cardiac regeneration, it would be highly desirable to reduce genetic manipulation or avoid the use of viral vectors. Here we report an efficient method of direct reprogramming of mouse fibroblasts into cardiomyocytes through a cardiac precursor-like stage rather than a pluripotent state. Importantly, mouse fibroblasts could be converted to cardiomyocytes by a non-viral approach, and these exosome-mediated cardiomyocyte-like cells spontaneously contract, express cardiomyocyte-specific markers, and exhibit typical cardiac calcium flux and electrophysiological features. Our approach may be a simple and safe method to suggest a pathway that can be a step closer to clinical application. |
