| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Polyacrylamide gel stabilization electrophoresis : Alpha-helix stabilization via copolymerization |
| 초록 |
Our work reports on the development of a novel strategy to stabilize bioactive alpha-helical peptides into a polyacrylamide hydrogel network. We synthesized an RNA binding peptide derived from the human immunodeficiency virus type-1 (HIV-1) Rev protein and two acryloyl groups were added to the peptide. We named it "bis-Rev". We prepared gels for electrophoresis in TBE buffer at 4 ℃. We confirmed that the bis-Rev are covalently captured in polyacrylamide gel by comparing acryloylated or non-acryloylated peptides on gel electrophoresis. Additionally, RRE mutant was used to confirm the specificity of the RNA-peptide binding. In conclusion, we developed a novel method for stabilizing alpha-helical peptides by copolymerization within a polyacrylamide gel. |
| 저자 |
한상헌, 임용범
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| 소속 |
연세대 |
| 키워드 |
peptide; polyacrylamide gel; alpha helix stabilization; copolymerization
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| E-Mail |
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