| 초록 |
In this study, we hypothesized that biomimetic nanotopographical features have enhanced cell recruitment, migration and differentiation from intact cells without additional stimulation. We designed a biodegradable and transplantable nanopatterned patch using simple solvent casting and capillary force lithography. We confirmed that biodegradable nanopatterned patch (BNP) accelerated migration of osteoblast according to orientation of patterned direction. These highly aligned osteoblasts might contribute to in vitro osteogenic differentiation such as ALP activity, mineralization and calcium deposition compared to the biodegradable flat patch (BFP). To demonstrate bone defect healing by guidance of BNP in vivo, we implanted nanopatterned patches on critical size defect of mouse calvarial or tibia bone defect and analyzed to use microcomputed tomography and histology. The BNP with biophysical guidance should be suitable utilized for tissue regeneration by accelerated intact cell migration. |
