| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.363 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Semi-Interpenetrating Polymer Networks Composed of Silk Fibroin and Poloxamer Macromer for Wound Dressing Application |
| 초록 | Introduction Silk fibroin (SF) were designed to utilize as a matrix for wound dressing by many researchers because the SF is biocompatible. However, the dry SF sponge itself is brittle although its application to wound dressing requires sufficient mechanical strengths. Improvements in mechanical properties of SF have been sought by blending with other synthetic or natural polymers1. In our system, poloxamer-407 macromer having an acrylated-terminated PEO derivative, was chosen as blending material and crosslinked in the presence of SF to form the semi-interpenetrating polymer networks (SIPNs). Poloxamer-407 cleans the wound of tissue detritus due to its surfactant nature and can significantly increase the rate of wound healing possibly by stimulation of endogenous production of epidermal growth factor2. Objectives : to investigate the properties of SIPNs composed of SF and poloxamer macromer and assess the potential as wound dressing material. Experimental Poloxamer 407 was end-capped with acrylated groups to form a polymerizable macromer. The sponge-type of SIPNs was obtained by irradiation of the mixtures of SF, poloxamer macromer and photoinitiator, followed by freeze drying. The thermal and mechanical properties of the SIPN hydrogels as well as their swelling behaviors were studied by means of differential scanning calorimetry, compressive modulus measurement, and gravimetric method, respectively. The morphology and crystalline structure of these SIPN hydrogels were also investigated by scanning electron microscopy (SEM) and wide-angle diffractometry, respectively. Conformational change of SF by formation of SIPNs with poloxamer was investigated using a FT-IR. Finally, their effectiveness in burn wound healing using BALB/c mice in vivo was examined. Results and discussion Obtained SIPN hydrogels were porous and their pores were well interconnected throughout the scaffold matrix. The Tms of poloxamer in the SIPNs were lower than that of poloxamer itself by the incorporation of SF and increased slightly with the amount of poloxamer macromer. Meanwhile, the Tds of SF in the SIPNs and blend did not show obvious difference in comparison with that of SF itself, and were not changed with SIPN composition. The resistance of SIPNs hydrogel against compression was much higher than that of SF itself or SF/poloxamer blend and increased with the poloxamer content. The equilibrium water content of SF was remarkably increased from 44 to 95 wt.-% by formation of SIPN with poloxamer due to the hydrophilicity of poloxamer. The crystallinity of SF/poloxamer SIPNs showed composition dependence and decreased from 28.2 % for SF/poloxamer (2/4) SIPNs to 22.5 % for SF/poloxamer (2/2) ones. The morphology of SIPN hydrogels were also affected by SIPN hydrogel composition and freezing temperature. Conformational change of SF from random coil to β-sheet structure was accelerated by formation of SIPN with poloxamer. In vivo test, granulosus tissue formation and wound contraction for SF/Poloxamer and DHEA-impregnated SF/Poloxamer wound dressing were faster than any other groups. From these results, SF/poloxamer SIPN hydrogels will be expected to be useful for wound dressing application. References 1. H.Y. Kweon, S.H. Park, J.H. Yeo, Y.W. Lee, and C.S. Cho, J Appl Polym Sci, 80, 1848 (2001). 2. R.M. Nalbandian, J Biomed Mater Res, 21, 1135 (1987). |
| 저자 | 유미경1, 권해용2, 이현철3, 박성식3, 조종수1 |
| 소속 | 1서울대, 2농업과학기술원 잠사곤충부, 3전남대 |
| 키워드 | Silk fibroin; Poloxamer macromer; Semi-interpenetrating networks (SIPNs); Wound dressing |
