| 초록 |
Small interfering RNAs (siRNAs) have been used to selectively regulate the gene expression by a sequence specific mRNA cleavage. To this end, a variety of viral vector and non-viral vector such as adenovirus, retrovirus, cationic polymers, lipids have been investigated for efficient intracellular delivery of siRNAs into the cells. However, the current delivery systems had multiple unwanted problems of cellular toxicity, immune response, and non-specificity towards tissue and cells. In this study, we introduced variety of ligands on siRNAs, which can enhance the cellular uptake and specific targeting. In addition, we designed well-defined three dimensional nucleic acid nanoparticles to further achieve efficient gene silencing in vitro as well as overcome the current problems of conventional cationic gene delivery systems. Two kinds of nucleic acid nanoparticles with the shape of tetrahedron and holiday junction were prepared by a programmable molecular self-assembly. Various ligands on these structured DNA nanoparticles have shown different cellular uptake and dose dependent gene silencing. |
