| 학회 |
한국공업화학회 |
| 학술대회 |
2006년 봄 (05/12 ~ 05/13, 전북대학교) |
| 권호 |
10권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Localization and characterization of validamycin biosynthetic gene cluster from Streptomyces hygroscopicus var. limoneus KTCC 1715 (IFO 12704) |
| 초록 |
The validamycin biosynthetic gene cluster was localized within 37-kb region in Streptomyces hygroscopicus var. limoneus KTCC 1715 (IFO 12704) using a pair of degenerated PCR primers designed from the sequence of AcbC, 2-epi-5-epi-valiolone synthase in the acarbose biosynthesis. The cosmid clone of pJWS3001 harboring the 37-kb DNA region conferred validamycin-accumulation to Streptomyces lividans, indicating that the entire cluster of validamycin biosynthesis had been isolated. Gene-inactivation and in vitro characterization studies elucidate validamycin biosynthetic route, which provides excellent opportunities to engineer the pathway for industrial application. |
| 저자 |
권형진 |
| 소속 |
명지대 |
| 키워드 |
validamycin; Streptomyces hygroscopicus; biosynthesis
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| E-Mail |
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