| 초록 |
We developed synthetic sRNAs system to identify and inhibit the expression of target genes. Among 101 sRNA scaffolds, three candidates were selected, and MicC scaffold which is a sRNA in Escherichia coli was superior than others. To make synthetic sRNA target mRNA, OmpC-binding sequence was deleted and instead, transcription start sequence of target genes were inserted. This sRNA system was successfully repressed DsRed2 expression level in E. coli. Then, sRNA system was applied to metabolic engineering and successfully overproduced tyrosine and cadaverine in E. coli. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and MSIP through the NRF of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557); the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of MEST through the NRF of Korea] |
