| 초록 |
Recently, RNA-guided CRISPR technologies have been applied to microbial cell for rapid development of metabolic engineering in microbial cell factories. To prove the application of the CRISPR interference to microbial cell, we constructed the two-plasmid system of CRISPRi and applied to C. glutamicum for repressing its single gene or double genes. Repression of four-different single gene was successfully performed using the two CRISPRi vectors resulting in significant reduction of targeted gene mRNA expression. Furthermore, double targeted gene repression using CRISPRi resulted in an independent gene repression to each target gene simultaneously. For its utilization to industrial strain, we introduced two plasmid vectors of CRISPRi into citrate synthase (CS)-targeting DM1919 (l-lysine producer), resulting in the improvement of l-lysine yield by 1.39-fold than the parental DM1919. Thus, this study could be useful for rapid development of microbial cell factories of C. glutamicum. |
