| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
신진연구자 특별 심포지움 |
| 제목 |
Single Molecule DNA Elongation in Nanoslit |
| 초록 |
Nanofluidic molecular confinement offers new routes for arraying large DNA molecules, enabling single-molecule schemes aimed at the acquisition of sequence information from individual DNA molecules. Such schemes can rapidly advance to become platforms capable of genome analysis. Here we demonstrate that PDMS nanoslits elongate DNA molecules by altering DNA stiffness. We also developed a complementary enzymatic labeling biochemistry that tags specific sequences on elongated DNA molecules within nanoslits that are imaged via fluorescence resonance energy transfer. Collectively, these developments enable scaleable molecular confinement approaches for genome analysis. |
| 저자 |
조규봉 |
| 소속 |
서강대 |
| 키워드 |
DNA elongation; nanoslit; scaling relationship
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| E-Mail |
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