| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 |
13권 2호, p.1688 |
| 발표분야 |
생물화공 |
| 제목 |
A quantitative detection of clinically important pathogen based on capillary electrophoresis coupled with multiplex PCR |
| 초록 |
Rapid diagnosis of bacterial infections has been considered as important criteria for a patient management and an appropriate therapy of bacteria-induced disease in the early phase. Among several techniques, capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR has come into the spotlight due to its benefits such as high sensitivity, resolution and great reproducibility. In this study, we developed a novel technique for quantitative pathogen diagnosis by CE-SSCP coupled with multiplex PCR. PCR primers were designed for the optimal separation in CE-SSCP using a stochastic model. PCR conditions were then optimized in order to obtain linear correlation between peak area and the concentrations of the target pathogens. Consequently, we developed a two-step CE-SSCP diagnosis method; first step for identification and the second step for quantification. As a model system, 8 species of clinically important bacteria were simultaneously identified and individually quantified. The results illustrated the potentials of this method on pathogen diagnosis and high-throughput drug discovery. |
| 저자 |
신기원1, 황희성2, 정규열3
|
| 소속 |
1I-Bio Program, 2Dept. of Chem. Eng., 3POSTECH |
| 키워드 |
pathogen; CE-SSCP; Multiplex PCR; rapid diagnosis
|
| E-Mail |
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| 원문파일 |
초록 보기 |
