| 초록 |
A high throughput toxicity biosensor has been designed and constructed using recombinant Escherichia coli cells, containing stress specific promoters (recA, fabA, grpE or katG) or constitutive promoters (lac) fused to luciferase genes originating from Vibrio fisheri. These genetically engineered cells were immobilized in 96 well plates. By optimizing cell immobilization conditions and the strains’ response specificity to toxic chemicals, bioluminescent outputs decreased or increased dose-dependently upon adding test chemicals. Phenolics were chosen to evaluate the correlation between the LD 50 and the EC 50 (GC2) or EC120 (DPD2540-fabA::lux) of D. magna and E. coli, respectively. Toxicity data obtained from constitutive strains by bioluminescent level decrements were compared with the results from D. magna as a standard. This novel high throughput toxicity biosensor can be implemented to investigate the toxicity of any other soluble materials, and can be used as a standardization tool for the evaluation of toxicity. |
