| 초록 |
An accurate, precise, and sensitive LC–MS/MS method was developed for the simultaneous determination of IMP and its metabolite, XAN, in rat plasma and urine. The analytes were determined by multiple reaction monitoring operated in the positive ESI mode. The analytes were eluted on an Acquity UPLC BEHC18 column (50 mm × 2.1 mm, 1.7 µm) by 0.1% formic acid solution and 0.1% formic acid in MeOH (0.3 mL/min). The run time was 6 min per sample and injection volume was 5 µL. The method had a lower limit of quantification of 0.25 ng/mL for IMP (plasma and urine), and 1 ng/mL for XAN (urine). Linear calibration curves were fitted over a range of 0.25–1000 ng/mL for IMP, and 1–1000 ng/mL for XAN (R ≥ 0.995). The method was then applied to determine PKs of IMP in rat plasma and, for the first time, the metabolite kinetics of IMP to XAN in rat urine after IMP administration. |
