| 학회 |
한국공업화학회 |
| 학술대회 |
2009년 가을 (10/15 ~ 10/16, 서울산업대학교 내 서울테크노파크) |
| 권호 |
13권 2호 |
| 발표분야 |
생물공학(포스터) |
| 제목 |
Enantioconvergent biotransformation of (R)-4-chrolopheny-1,2-enthandiol by highly active epoxide hydrolase |
| 초록 |
In order to enhance the activity of the recombinant epoxide hydrolase of Caulobacter crescentus(CcEH), the EH gene was expressed in the presence various molecular chaperone and foldase. Escherichia coli harboring various plasmids containing chaperones was used as a host. The specific activity of CcEH was the highest when the gene was expressed with pTf16 plasmid containing tig gene. We carried out enantioconvergent hydrolysis of racemic 4-chlorostyrene oxide for the preparation of enantiopure (R)-4-chrolopheny-1,2-enthandiol. Chiral (R)-4-chrolopheny-1,2-enthandiol with enantiopurity more than 90% was obtained with more than 50% yield. |
| 저자 |
민지영, 이은열
|
| 소속 |
경희대 |
| 키워드 |
Enantioconvergent; (R)-4-chrolopheny-1; 2-enthandiol; epoxide hydrolase; chaperone
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| E-Mail |
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