| 초록 |
The three dimensional scaffolds which can guide cells to grow, synthesize extracellular matrix, and thus facilitate the formation of functional tissues and organs are widely utilized for the tissue engineering. However, the non-uniform cell distribution or cell necrosis due to the insufficient oxygen diffusion into the center of the scaffold (cell carrier) have been considered as critical limitation for the formation of 3D tissues/organs. In this study, we developed oxygen-loaded hollow microparticles as a cell carrier which can allow continuous oxygen release for sufficient period, and thus provide appropriate environment for cell survival/growth. The viability and hypoxia-inducible gene expression of the cells seeded on the hollow microparticle (in hypoxia) were evaluated (in vitro). And cell distribution and tissue responses in cell/oxygen-loaded hollow microparticle were also investigated using an animal model (nude mouse, subcutaneously injection). |
