| 초록 |
The nervous system plays an important role in the body to control and regulate various body organ activities. For the observation of highly mature and extended axons and dendrites of the primary neurons, long-term culture of the primary hippocampal neuron cells is of critical importance, which had been shown to be extremely challenging. Here, we introduce a stabilized polymer with acetylcholine-like functionalities conformally coated on cover glass. The polymer film was coated via initiated chemical vapor deposition (iCVD), ensuring its high purity, and thus the biocompatibility to primary neuron culture. The neuron cells cultured on glass plates with iCVD coating showed rapidly and extensively extended axon branches and dendrites than the cells cultured on PLL-coated glass after a 8 day culture. Furthermore, the uniformly-distributed neuron cells survived over 90-day culture on the polymer-coated glass, confirming the long-term culture capability of the polymer-modified glass plates. |
