| 초록 |
To understand protein functions, a number of researches and binding chemistries have been studied to immobilize small molecules, peptides, and proteins in a microarray format for high-throughput analysis. The growing range of applications for peptide arrays proves they have emerged as a powerful proteomic technique because many enzymatic processes, especially kinases, can be studied using peptides as model substrates for applications in biological or pharmaceutical researches. The present investigation provides a practical peptide chip for efficient evaluation of kinase activity to reduce steric effect making protein layer with a recombinant strategy. The peptide chip for protein kinases is convenient and effective to use and we expect this method to be valuable for protein chip using peptides or small molecules. [Our work described in this paper was supported by the Center for Ultramicrochemical Process Systems (CUPS)]. |
