| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Mucoadhesive and pH-sensitive thiolated Eudragit microspheres for oral delivery of Pasteurella multocida antigens containing dermonecrotoxin |
| 초록 |
Oral vaccination is an attractive route of vaccine delivery. However, low oral bioavailability, degradation and short residence time of antigen at target site are the prime problems. Pasteurella multocida antigens containing dermonecrotoxin (PMT) is a major virulence factor of atrophic rhinitis in swine. To overcome these problems, PMT-loaded mucoadhesive and pH-sensitive thiolated Eudragit microspheres (PMT-loaded TEMS) were prepared. Modification of Eudragit® L-100 was done by reaction with L-cysteine HCl. PMT-loaded TEMS were prepared by double emulsion (w/o/w) method. About 8.9 mol-% of cysteine was introduced to the Eudragit. PMT-loaded TEMS were spherical with smooth surface and average particle sizes were 5.2 m. The TEMS provided higher vaccine loading content and more vaccine was released at pH 7.4 than at pH 1.5. Immune stimulation in Raw 264.7 by PMT-loaded TEMS will be reported. The TEMS will provide a promising delivery system for oral administration of vaccine. |
| 저자 |
모하매드 아리풀, 강호림, 전희선, 로히다스, 유한상, 윤철희, 조종수
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| 소속 |
서울대 |
| 키워드 |
Thiolated Eudragit microspheres; Pasteurella multocida dermonecrotoxin; Oral vaccination; Macrophage; Tumor necrosis factor; Nitric oxide
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| E-Mail |
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