| 학회 | 한국화학공학회 |
| 학술대회 | 2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 | 19권 2호, p.1542 |
| 발표분야 | 생물화공 |
| 제목 | Analysis of Y-shaped DNA preparation conditions using optical density measurement |
| 초록 | In the field of bioengineering, DNA has gathered enormous attention due to its inherent advantages. It can be built into a programmed structure using diverse protein tools in nano-scale precision. So there emerged Y-shaped DNA (Y-DNA), composed of three oligonucleotides. Its anisotropicity, ability to be modified and possibility to form sophisticated structures enabled Y-DNA to be utilized as a powerful coding material. In this study, we assessed three conditions which may influence on the Y-DNA preparation efficiency: Concentration, volume and dilution ratio. After Y-DNA synthesis, optical densities of the products were measured and compared with theoretical optimum values which were calculated by using Matrix Laboratory (MATLAB) program. Through such analysis, it will lead to a more effective preparation of Y-DNA. Acknowledgement: This work was supported by a grant from the Korean Health Technology R&D Project of the Ministry of Health & Welfare of the Republic of Korea (Grant No. A110552) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant No. 2013R1A1A2016781) |
| 저자 | 박경수1, 최정우1, 엄숭호2 |
| 소속 | 1서강대, 2성균관대 |
| 키워드 | DNA; nanotechnology; DNA block |
| 원문파일 | 초록 보기 |
