초록 |
Cells communicate with adjacent cells and surrounding microenvironments to maintain their viability and phenotype as well as to develop tissues. Specifically cell-cell and cell-matrix interactions are important for chondrocyte growth and development, because cartilage is composed with one type of cells and there is no vessel to transfer nutrient and gas. In this study, cell-matrix and cell-cell interactions of chondrocytes were controlled in vitro using RGD-modification and cell pellet formation, respectively. Chondrogenic differentiation of ATDC5 cells were evaluated using alginate hydrogels by histological and immunohistochemical analysis and quantification of chondrogenic gene expression levels. Considering cell-matrix and cell-cell interactions together in design of synthetic extracellular matrices could be critical to successfully regenerate cartilage tissue in the body. |