| 학회 |
한국재료학회 |
| 학술대회 |
2013년 봄 (05/23 ~ 05/24, 여수 엠블호텔(THE MVL)) |
| 권호 |
19권 1호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Three–dimensional fibrous BCP-PCL/GEL composite scaffold for BMP-2 delivery |
| 초록 |
Recombinant human bone morphogenetic protein-2(rhBMP-2) coated biphasic calcium phosphate(BCP)-polycaprolactone(PCL)/gelain(GEL) composite fibrous scaffolds were fabricated successfully using electrospinning method. The electrospinning process to incorporate BCP nanoparticles into PCL/GEL scaffolds yielded a porous matrix and produced fibers in the diameter range from a few micros to nanometers. Then, rhBMP-2 was coated on these scaffolds for enhanced to bone inductivity. These rhBMP-2 coated BCP-PCL/GEL scaffolds were compared with BCP-PCL/GEL membranes by mechanical properties and biological behaviors. These scaffolds were characterized by scanning electron microscopy (SEM), electron dispersion spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectra. The morphology of these scaffolds showed highly porous and interconnected structure after cross-linking and coating. Compared with BCP-PCL/GEL scaffold, the rhBMP-2 coated BCP-PCL/GEL scaffold exhibited excellent biocompatibility, cell proliferation and cell adhesion behavior resulted in the MTT assay (1, 3, 7days) and immunofluorescence analysis using pre-osteoblast MC3T3-E1 cells. Based on these results, rhBMP-2 coated BCP–PCL/GEL membranes could be considered as potential bone tissue engineering applications. |
| 저자 |
Bo-ram Kim1, Nguyen Thuy Ba Linh2, Andrew Padalhin3, Young-Ki Min4, Hun-Mo Yang5, Byong-Taek Lee1
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| 소속 |
1Department of Biomedical Engineering and Materials, 2College of Medicine, 3Soonchunhyang Univ., 4Chungnam 330-930, 5Korea |
| 키워드 |
electrospinning; polycaprolactone; gelatin; BCP; BMP2; bone tissue engineering; bone regeneration
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| E-Mail |
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