| 초록 |
With increased importance of sensitive optical bio-imaging techniques, conjugated fluorophores are notably used to stain in a range of biomolecules with high quantum yield, fast emission rate, excellent photostability. Raman spectroscopy is also one of efficient tools to clarify the vibrations of molecules by utilizing multiplexes, but extremely weak signal limits the its application. Herein, we developed fluorescent and highly Raman-active conjugated structures, by increasing the Raman intensity by several orders of magnitude via resonance Raman scattering. In addition, fine-control of intramolecular conjugation by changing the accepting unit provide shift of absorption/emission spectra as well as Raman multiplexes, enabling multimodal probe. After co-precipitating the probes with polystyrene maleic anhydride which produces carboxylic acid on the polymer dot surfaces, further conjugation with target-specific biomolecules encourages the fluorescence/Raman dual imaging. |
