| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.946 |
| 발표분야 |
재료 |
| 제목 |
Sensitive and optimal detection of mutations in epidermal growth factor receptor via gold nanorods-quantum dots FRET biosensor |
| 초록 |
We report a simple, sensitive fluorescence assay for the detection of mutations in epidermal growth factor receptor (EGFR) by using gold nanorods. Almost of oncogenic mutations were located in exon 19 and exon 21 of EGFR gene from patients with non-small cell lung cancer (NSCLC). Gold nanorods with localized surface plasmon resonance (LSPR) peak at 600nm well caused fluorescence quenching with quantum dots (QD) on the basis of the fluorescence resonance energy transfer (FRET). The quenching efficiency is related to the spectral overlap of the GNR absoption and QD emission, distance between the two nanoparticles and probe length for hybridization. This study will be useful for sensitive and efficient detection of EGFR mutations in lung cancer. |
| 저자 |
안희영1, 강태경1, 윤경아2, 이강택1
|
| 소속 |
1연세대, 2국립암센터 |
| 키워드 |
gold nanorod; FRET; quantum dot; EGFR
|
| E-Mail |
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| 원문파일 |
초록 보기 |
