| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
(생물공학)시스템/합성생물학과 생체분자공학 최신기술 |
| 제목 |
3’UTR engineering to improve functional expression of insoluble enzymes in Escherichia coli |
| 초록 |
Functional expression of the catalytic enzymes in microbial cells is essential to produce chemical products via whole-cell biocatalysis. Thereby, 3’-untranslated region (3’UTR) engineering was investigated to improve solubility of the heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of the gene fragments containing putative RNase E recognition sites into the 3’UTR of the BVMO genes led to reduction of the corresponding mRNA level in E. coli BL21(DE3). However, the amount of BVMOs in soluble fraction was remarkably enhanced resulting in a proportional increase of in vivo catalytic activity. The increase of whole-cell biocatalytic activity notably correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3’UTR. For instance, 12-keto oleic acid biotransformation activity of the BmoF1 of Pseudomonas fluorescens DSM50106 was linear to the number of RNase E cleavage sites in the 3’UTR. |
| 저자 |
박진병 |
| 소속 |
이화여자대 |
| 키워드 |
3’UTR engineering; functional gene expression; Escherichia coli
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| E-Mail |
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