| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Articular Cartilage Tissue Engineering with PLGA/Keratin scaffolds |
| 초록 |
Keratins are the intermediate filament proteins that form a dense meshwork of filaments throughout the cytoplasm of epithelial cells. We prepared PLGA and PLGA/Keratin scaffolds using solvent casting/salt leaching method. Chondrocytes were isolated from the articular cartilage of rabbit and cultured. Cell proliferation activity was by MTT assay. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed at 1, 2 and 3 weeks. The cell viability and the quantity of collagen and sGAG were better PLGA/Keratin scaffolds than PLGA scaffolds. Specific mRNA, collagen type II and aggrecan, for chondrocyte expressed significantly highly in PLGA/keratin scaffold while dedifferentiation marker, collagen type X, did not appear. This study suggests that PLGA/Keratin scaffold may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage. Acknowledgements This research was supported by SCRC (SC3100). |
| 저자 |
정수현1, 김순희1, 임지예1, 백미옥1, 최진희1, 홍현혜1, 소정원1, 이종문1, 강길선1, 이해방2
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
Keratin/PLGA Scaffold |
| E-Mail |
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