| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.460 |
| 발표분야 | 생물화공 |
| 제목 | High-level production of poly(3-hydroxybutyrate) by amplifying glycolytic pathway enzymes in recombinant Escherichia coli |
| 초록 | Since the production cost of poly(3-hydroxyalkanoates) [PHAs] is mainly composed of the cost of carbon source, it is important to produce PHAs with high yield on carbon source to reduce production cost of PHAs [1]. Escherichia coli strain has been most promising host strain for the efficient production of PHAs. Recent studies on the biosynthesis of poly(3-hydroxybutyrate) [P(3HB)] in E. coli have revealed that the level of acetyl-CoA and NADPH is the most important factor determining yield of P(3HB) on carbon source [2]. E. coli has been metabolically engineered to make more acetyl-CoAs available for the P(3HB). In this study, we report that the amplification of glycolytic pathway enzymes support the efficient production of P(3HB) in recombinant E. coli. [This work was supported by the National Research Laboratory Program of the Korean Ministry of Science and Technology.] REFERENCES [1] Lee, S. Y. 1996. Biotechnol. Bioeng. 49: 1-14. [2] van Wegen et al. 2001. Biotechnol. Bioeng. 74: 70-80. [3] Madison, L. L. & G. W. Huisman. 1999. Microbiol. Mol. Biol. Rev. 63: 21-53. |
| 저자 | 송애진, 이상엽, 박시재 |
| 소속 | 한국과학기술원 생명화학공학과 |
| 키워드 | poly(3-hydroxybutyrate); glycolytic enzymes; recombinant E. coli |
| , , |
