| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.435 |
| 발표분야 | 생물화공 |
| 제목 | Transcriptome profiling of Escherichia coli fadR mutant |
| 초록 | The identification of regulatory circuit of global regulators is a prerequisite for construction of industrially useful strains. In this study we examined the regulatory circuit of FadR, which is one of the global regulators in Escherichia coli, at transcriptional level using DNA microarray experiment. In Escherichia coli expression of the genes of fatty acid metabolism is regulated by FadR. It negatively regulates the expression of fatty acid degradation-related genes, and positively regulates the expression of fatty acid biosynthetic genes by binding the promoter region of corresponding genes. The global effect of fadR knockout is identified by transcriptome profiling in the presence of glucose or oleic acid as a carbon source. In the presence of glucose, 190 genes are up-regulated (fold change ratio > 2.0) and 270 genes are down-regulated (fold change ratio < 0.5) in fadR knockout mutant. With oleic acid as a carbon source, 78 genes are up-regulated and 145 genes are down-regulated in fadR knockout mutant. With glucose, more genes are down or up-regulated than with oleic acid. Acknowledgement This work was supported by the Korean Systems Biology Research Grant (M10309020000-03B5002-00000) from the Ministry of Science and Technology. Hardware support by the IBM-SUR program is greatly appreciated. |
| 저자 | 박진환, 김병훈, 최종현, 이상엽 |
| 소속 | 한국과학기술원 |
| 키워드 | FadR; transcriptome profiling |
| 원문파일 | 초록 보기 |
