| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
나노_포스터 |
| 제목 |
Nanoplasmonic biosensors for detecting dual bio-signatures of circulating tumor DNA (ctDNA) |
| 초록 |
Circulating tumor DNA (ctDNA) with tumor-specific mutation and methylation are promising biomarkers for noninvasive cancer assay. However, existing methods for ctDNA detection are limited to genetic mutations. Here we present a strategy for ultrasensitive detection of tumor-specific mutations and methylation of ctDNA based on localized surface plasmon resonance (LSPR) and the coupling plasmon mode of gold nanoparticles (AuNPs). Peptide nucleic acid (PNA) is used as a probe to capture and enrich ctDNA. The exposure of PNA-probed AuNPs to target ctDNA generates the primary response of LSPR-peak shift. Immunogold colloids are exploited as methylation detectors and plasmon coupling enhancers for secondary response. LSPR-peak shift is increased approximately 107% upon immunogold colloids binding to two methylcytosines, compared to the primary response. These results demonstrate that the sensor can simultaneously detect the hot-spot mutation and epigenetic changes on the ctDNA. |
| 저자 |
Nguyen Hung Anh, 전명진, 심상준, 최홍일
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| 소속 |
고려대 |
| 키워드 |
ctDNA; AuNPs; LSPR; DNA methylation
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| E-Mail |
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