| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 | 15권 2호, p.1586 |
| 발표분야 | 생물화공 |
| 제목 | Engineering a novel synthetic pathway for butanol production in Escherichia coli |
| 초록 | We engineered the novel synthetic pathway for the production of butanol in Escherichia coli by using 2-ketoisovalerate as an intermediate. Based on the previously constructed L-valine producing strain of E. coli, in which all the known negative regulations by L-valine were removed and the carbon flux towards L-valine formation was increased, the novel pathway for the biosynthesis of butanol from 2-ketoisovalerate, the direct precursor of L-valine, was further overexpressed. The resulting engineered E. coli strain was able to produce 118 mg/L butanol by microaerobic batch culture. These results suggest that an efficient production of butanol is possible by properly assembling the synthetic metabolic pathways in E. coli. [This work was supported by the Korea-Australia collaborative research project on the development of platform technologies for the production of biobutanol based on renewable biomass (N02071165) from the Korean Ministry of Knowledge Economy. Further supports by the LG Chem Chair Professorship and Microsoft are appreciated]. |
| 저자 | 박진환, 최형석, 이종민, 이상엽 |
| 소속 | KAIST |
| 키워드 | biobutanol; 2-ketoisovalerate; Escherichia coli; metabolic engineering |
| 원문파일 | 초록 보기 |
