| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.408 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic engineering of E. coli for the enhanced production of L-valine based on comparative transcriptome analysis |
| 초록 |
Escherichia coli L-valine production strain was constructed by rational design. L-valine production strain was constructed by releasing two regulatory mechanisms, feedback inhibition and attenuation. Two amino acids alterations were introduced into ilvH which is subject to feedback inhibition by using site-directed mutagenesis. The leader region of ilvGMEDA and ilvBN operon which is involved in attenuation was changed with the strong tac promoter by homologous recombination. Further improvement of the L-valine production strain was achieved by knocking out ilvA, leuA and panB genes thus making more substrate available for L-valine biosynthesis. Transcriptome analysis was used to identify the physiology at mRNA level during the biosynthesis of L-valine. And we also achieved improvement of L-valine production strain based on transcriptome profiling.[This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. M10309020000-03B5002-00000). Further supports by LG Chem Chair Professorship, Microsoft and IBM SUR program are appreciated.] |
| 저자 |
박종명, 박진환, 이광호, 이상엽
|
| 소속 |
한국과학기술원 생명화학공학과 |
| 키워드 |
Metabolic engineering; E. coli; L-valine
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| E-Mail |
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| 원문파일 |
초록 보기 |
