| 초록 |
Biological conversion of glycerol into valuable biochemicals has attracted lots of attentions because glycerol is by-product of the biodiesel production. One of these chemicals is 3-hydroxypropionic acid (3-HP) that can be produced from glycerol via two enzymatic reactions: glycerol to 3-hydroxylpropanal (3-HPA) via glycerol dehydratase and 3-HPA to 3-HP via aldehyde dehydrogenase. Biological processes using the two reactions have been developed, and the productivity of more than 0.86g/L/hr was reported. However, improvement in the productivity is needed to move to a next phase of commercialization, and some issues have been raised. One of them is the toxicity of 3-HPA. An approach to solve the problem is to develop a highly active aldehyde dehydrogenase, and the process can be operated at a low concentration of 3-HPA. In this presentation, we report the results of engineering the substrate specific of an aldehyde dehydrogenase, α-ketoglutaric semialdehyde dehydrogenase toward 3-HPA. |
