| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System improves osteogenic differentiation of Human Mesenchymal Stem Cells |
| 초록 |
In tissue engineering, Human mesenchymal stem cells(hMSCs) are known to have a great potential. However, current application of hMSCs in therapy has challenges because it is difficult to control that cell in vivo such as differentiation to a target cell type. One of the major difficulties for genetic modification of hMSCs is the lack of an efficient and safe gene delivery system. A conjugated catechol-derivatized hyaluronic acid(dopa-HA) can stabilize biocompatible calcium phosphate(CaP)-based nanoparticles. This complex is used as a carrier for gene transfection to hMSCs for enhanced differentiation. As HA show a specific interaction with CD44 of bone marrow-derived hMSCs, dopa-HA/CaP is used for significantly higher transfection in hMSCs than branced polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of micro RNA 148b and a plasmid DNA encoding bone morphogenetic protein 2 by dopa-HA/CaP achieved remarkably improved osteogenic differentiation of hMSCs. |
| 저자 |
박채은, 임수연, 정지훈, 김다훤, 황위보, LYUSIYAN, 김병덕
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| 소속 |
성균관대 |
| 키워드 |
drug delivery system |
| E-Mail |
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