| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Articular Cartilage Regeneration using Hyaluronic Acid Loaded PLGA Scaffold by Emulsion Freeze-drying method. |
| 초록 |
PLGA and hyaluronic acid(HA) has been widely used as biocompatible scaffolds materials to regenerate tissue. HA loaded PLGA scaffolds were prepared by a emulsion freeze-drying method. The chondrocytes were seeded on the HA-PLGA scaffolds and measured by MTT assay. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed. In MTT assay result, scaffolds containing HA were higher cell viability then only PLGA scaffolds. Although collagen, sGAG, mRNA, collagen type II and aggrecan were greater than HA-PLGA scaffolds. When we cultured cartilage cell of rabbit in vitro, we observed better to keep the characteristic of cartilage cell in the HA-PLGA scaffolds than that PLGA scaffolds. This study suggests that HA/PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage. Acknowledgements This research was supported by KMOHW (0405-BO01-0204-0006) |
| 저자 |
정수현1, 김순희1, 임지예1, 홍현혜1, 소정원1, 백미옥1, 이종문1, 강길선1, 이해방2
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
HA/PLGA Scaffold |
| E-Mail |
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