| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
NP regeneration on PLGA scaffold penetrated SIS gel in vitro |
| 초록 |
Small intestinal submucusa(SIS) has been used as biomaterials possessing cytokines and biocompatibility. PLGA has strength physical property and biodegradable. PLGA scaffolds impregnated with SIS gel(PLGA/SIS) were prepared as novel intervertebral disc(IVD) replacement by the technique of tissue engineering. PLGA(90K) scaffolds were made by solvent casting/salt leaching method using NaCl(size:90~600 µm). These scaffolds immersed into 1% SIS gel, lyophilized, and crosslinked. Nucleus pulposus was isolated from the IVD. We confirmed cell viability in scaffold. The degree of collagen synthesis was tested through hydroxyproline assay and the formation of disc tissue was evaluated through histology. We chose porogen size of 250~355 µm according to cell viability and hydroxyproline assay. PLGA/SIS scaffold showed good initial attachment, high collagen synthesis, cell viability, and good expression of proteoglycan and type II collagen.- This work was supported by SCRC(SC3100). |
| 저자 |
김순희1, 소정원1, 백미옥1, 김수미1, 이종문1, 이해방2, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
nucleus pulposus; SIS; PLGA
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| E-Mail |
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