| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Reactive Poly(Pentafluorophenyl Acrylate) Brush Platforms for Protein Immobilization |
| 초록 |
Antibody-antigen precipitation or immunoprecipitation experiments are routinely performed by biologists to isolate specific antigens and also to identify their interactors from complex protein mixtures. However, the conventional approach involving agarose supports often suffers from highly nonspecific binding and antibody contamination. To overcome these disadvantages, we prepared poly(pentafluorophenyl acrylate) (poly(PFPA)) brushes on silica beads via the surface-initiated RAFT polymerization. Poly(PFPA) is known to have high reactivity with amine functional groups that offers facile approach to realize diverse functions starting from the same platforms. These platforms could also circumvent the conventional problems by covalently immobilizing antibodies together with amino-terminated polyethylene glycols on the brushes. Considering their versatility and convenient features, we expect that the poly(PFPA) platforms could be utilized as an alternative to traditional agarose supports. |
| 저자 |
손현주, Sheng Li, 김유식, 김빛내리, 차국헌
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| 소속 |
서울대 |
| 키워드 |
Active Ester Polymer; Polymer Brush; Post-Modification; Protein Immobilization
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| E-Mail |
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