| 초록 |
Gellan gum (GG) is an exopolysaccharide produced by Sphingomonas elodea. In this study we prepared three dimensional hydrogel of GG reacted with 1-ethyl-3-(3-di-methylaminopropyl) carbodiimide (EDC). From the FT-IR analysis, the mechanism of EDC cross-linking in the GG was the condensation reaction between the carboxyl and hydroxyl group. Because polysaccharide hydrogels normally possess poor mechanical strength, the results obtained in this study indicate that GG is sutiable for potential applications in tissue regeneration. In vitro biocompativility tests, hydrogel exhibited nontoxic effects. When implanted in to rat subcutaneous tissue, implantation showed that hydrogel caused slight inflammation in the first few days after operation. In the tissue engineering, cross-linked GG demonstrated good physical properties and biocompatibility, and has a great potential for future use. This research was supported by WCU (R31-20029) and MBC (0405-BO01-0204-0006). |
