| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.369 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Mechano-active tissue engineered vascular graft: In vitro study of vascular cell culture on Poly(L-lactide-co-ε-caprolactone) scaffolds |
| 초록 | Prosthetic vascular grafts such as PTFE and Dacron have been applied as a large-diameter vessel substitute (＞6mm) for the treatment of peripheral vascular disease. However, smaller ones than 6 mm in the diameter limited due to mechanical failings such as compliance insufficiency and biological failings such as thrombus/occlusion. We have developed a tissue engineered small-diameter vascular grafts using elastic and biodegradable 3D-porous tubular scaffolds, which are made of poly(L-lactide-co-ε-caprolactone) (PLCL). Vascular smooth muscle cells (VSMCs) were isolated from rabbit aortas and seeded onto PLCL scaffolds. After 1week static culture, the grafts were cultured for 7weeks under pulsatile radial strain . Scanning electron microscope(SEM) images of the cultured graft showed aligned SMCs parallel to pulsatile radial direction. The alignment was similar to a typical structure of native SMCs in blood vessel. The expression of SM alpha-actin and elastin were up-regulated in the VSMCs cultured dynamically compared with those cultured statically. On the other hand, the pulsatile radial strain did not affect collagen synthesis in VSMCs cultured on PLCL scaffods. Taken together, these results indicate that pulsatile stress have an influence on extracellular matrix synthesis and cell differentiation in primary cultured VSMCs. Fig. 1. In vitro Cells culture in a bioreactor 참고문헌 1. S, I, Jeong, S, H, Kim, Y, H, Kim, Y, M, Jung, J, H, Kwon, B, S, Kim, Y, M, Lee, J. Biomater. Sci. Poltmer Edn, 15, 645-660 (2004) 2. S, I, Jeong, J, H, Kwon, J, I, Lim, S, W, Cho, Y, M, Jung, W, J, Sung, S, H, Kim, Y, H, Kim, Y, M, Lee, B, S, Kim, C, Y, Choi, S, J, Kim, Biomaterials (2004) |
| 저자 | 임진익1, 김수현1, 김수자2, 성원준1, 권재현1, 김상헌1, 김영하1 |
| 소속 | 1한국과학기술(연), 2경희대 |
| 키워드 | Smooth muscle cells; Poly(lactide-co-caprolactone); Elastic scaffold; Vascular grafts; Tissue engineering; Pulsatile radial strain |
