| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Systematic engineering of L-tryptophan aptamer to construct ligand-responsive RNA-based sensor |
| 초록 |
The Potential of the riboswitch which implements cis-regulatory mechanism has been highlighted for monitoring various molecules in vivo. Several parameters of riboswitch should be considered to apply it in user-defined condition. However, it is not straightforward to tune the parameters since detailed numeric information between the concentration of the ligand and the strength of expression is required. Here, we demonstrate three strategies to optimize an artificial L-tryptophan riboswitch. We modulated the strength of the promoter or the copy number of the plasmid to optimize dynamic range and fold activation. Then tetA was utilized to amplify the response by tetracycline supplementation. Finally, a riboswitch variants were constructed using an aptamer with distinct affinity to L-tryptophan to adjust the operational range. All strategies could be easily applied to the optimization of other riboswitches owing to their simplicity. |
| 저자 |
김민선, 장성호, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Tryptophan; dose-response curve; Riboswitch; Synthetic biology
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| E-Mail |
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