| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 | 23권 1호, p.514 |
| 발표분야 | 생물화공 |
| 제목 | Production of recombinant cellobiose dehydrogenase from Phanerochate chrysosporium for enzymatic fuel cell |
| 초록 | Cellobiose dehydrogenases (CDH) structure consists of two distinct domains, flavin adenine dinucleotide (FAD) and cytochrome, which contains dehydrogenase and heme B respectively. Based on the enzyme structure, CDH has been reported with high attention in electrochemical applications such as enzymatic fuel cells (EFC) and biosensors. In this study, heterologous expression of the cdh from P. chrysosporium KCCM 60256 in Pichia pastoris X-33 was achieved for the reliable and efficient production of the enzyme. The anode and cathode of EFC system were modified with recombinant CDH (PcCDH) and laccase, respectively which were composed of graphite oxides immobilized on the modified Au surface. The reaction conditions of the EFC were a 10mM lactose solution at pH 4.5 in 0.1 M citrate buffer. The maximum power density obtained in this EFC with PcCDH was higher than the previosly results. The successful demonstration of this study suggests the potential development of EFC system based on the application of PcCDH. |
| 저자 | 최한석1, 김동섭1, 이주훈1, 이수권1, 김형렬1, 양지현1, 박철환2, 유하영3, 김승욱1 |
| 소속 | 1고려대, 2광운대, 3상명대 |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
