| 초록 |
Study of cell-cell interaction is significant in order to fully understand how the cellular functions are controlled. In order to study this in vitro, 3D environments similar to the extracellular matrix(ECM) need to be provided. In this research, we developed a co-culture system using a multifunctional micropatterned electrospun scaffold. The micropatterned scaffolds were produced by first obtaining an electrospun fiber matrix followed by micropatterning with poly(ethylene glycol)(PEG) using photolithography. The fibrous matrix provided a structure similar to the ECM to the cells and the PEG hydrogel functioned as barriers between each patterned wells. Cells only adhered to the fiber region due to the non-adhesiveness of PEG hydrogel thus creating a patterned cell culture which facilitates the control of cell behavior. Once the scaffolds were prepared, two different types of cells were seeded onto different scaffolds, respectively, and then stacked into layered form using a device. |
