| 초록 |
Exosomes are extracellular vehicles that contain a variety of proteins from cells. They can act as a potential biomarker for cancer detection due to the presentation of characteristic moieties originated from cells. Additionally, there is a higher frequency of polysaccharide on surface of exosomes. Thus, it could be suggested that lectins could bind with glycans expressed in exosomes as a substitute of conventional antibody. Thus, this study demonstrated the quantitative methods to determine the affinity between lectins and exosomes. The results indicate that Aleuria aurantia lectin (AAL) (binding with fucose) could bind strongly with exosomes compared with other lectins and has similar binding capacity to CD63 antibody. Furthermore, the PANC-1 exosomes could bind strongly with AAL as compared to those from other cells. In conclusion, AAL has a potential in detecting PANC-1 exosomes and further we will develop AAL-conjugated nanoparticles for diagnosis of pancreatic cancer. |
