| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO)) |
| 권호 |
40권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Interactions between DNA staining dyes on DNA particles for multicolor DNA microbeads |
| 초록 |
Recent advances in DNA technology have shown that DNA structures can be utilized for various fields including biological application. Although fluorescent labeling have been well used for monitoring the DNA structures in vitro/vivo, the conventional labeling agents have limitations in view of the cost-efficiency. Here we synthesized spherical DNA microparticles and stained the particles with two different DNA intercalating dyes. By staining the DNA balls with different methods, we investigated förster resonance energy transfer (FRET) and competition between the dyes on the DNA particles. The DNA particles showed different aspects of fluorescence intensities in accordance with the sequence and the staining methods. By controlling these properties, DNA particles can be designed as potent multicolor tracers for biological imaging and environmental applications. |
| 저자 |
이종범, 김지은
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| 소속 |
서울시립대 |
| 키워드 |
DNA particles; DNA technology; FRET
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| E-Mail |
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