| 초록 |
Circulating cell-free micro-ribonucleic acids are the most promising biomarkers for cancer diagnosis, which can be used to define relevant subtypes and treatment response. Herein, we developed a label-free, ultra-high sensitivity and selectivity multiplex biosensor using surface-enhanced Raman scattering (SERS) for the detection of specific miRNAs. A reproducible SERS signal was monitored via uniformly fabricated elastic head-flocked gold nanopillars. For multiplex detection, we selected target miRNAs associated with cancer in the metastatic state (miR-10b, miR-21, and miR-373) as model analytes. By using a complementary DNA probe platform and an ultra-high sensitivity plasmonic biosensor, we achieved label-free detection of target miRNAs and evaluated the correlation between the miRNA expression level and the Raman signal intensity. Through this process, we reached femtomolar (fM) detection limits (3.53, 2.17, and 2.16) for miR-10b, miR-21, and miR-373, respectively. |
