| 초록 |
Capillary electrophoresis on microchip-based genetic analysis systems are very useful because of rapid analysis, tiny sample/reagent consumption, and portability. capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is promising genotyping method due to its simplicity and versatility. The electrophoretic mobility variation comes from sequence-induced folded conformation difference of single strand DNA. Therefore, any DNA sequence variation cause shift in the mobility and can be detected upon electrophoresis in non-denaturing condition. However, the most critical limitation of CE-SSCP on microchip is low separation resolution resulting from short separation channel length. In this study, we translated CE-SSCP system into a microchip format and improved separation resolution via microchannel width control. |
