| 학회 |
한국고분자학회 |
| 학술대회 |
2022년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
47권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Morphology Characterization of Polyelectrolyte Complex Micelles Formed by PEGylated DNA and Polylysine |
| 초록 |
Nucleic acid condensation is a key process in various biomedical applications including gene delivery. Polycations such as polylysine (PLys) have been employed to condense negatively charged nucleic acids. However, the complexes showed poor stability near the isoelectric point. In this study, we demonstrate the condensation of DNA with PLys by using poly(ethylene glycol) (PEG) modified DNA. PEGylation of DNA produces anionic-neutral DNA-PEG block copolymers. They are then complexed with PLys to form polyelectrolyte complex micelles (PCMs) with a pseudo-neutral DNA/PLys core and neutral-hydrophilic PEG corona. PCMs formed with different PLys chain length are examined under varying N/P ratio conditions. The micelle size and dispersity are then correlated to PLys chain length. PCMs formed from long PLys show strong size and dispersity dependence on N/P ratio. When shorter PLys chains are used, the PCM size and dispersity remain small irrespective of the N/P ratio. |
| 저자 |
이광희, Sheng Li
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| 소속 |
한국과학기술원 |
| 키워드 |
Polyelectrolyte complex micelle; Morphology; DNA block copolymer
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| E-Mail |
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