| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Co-delivery of Akt1 shRNA and paclitaxel by thermo-sensitive conjugated linoleic acid-coupled poloxamer hydrogel |
| 초록 |
Akt is considered an exciting target for therapeutics. It is suggested that the reduction of Akt expression may increase the therapeutic efficacy of paclitaxel. In the present study, thermo-sensitive CLA-coupled poloxamer hydrogel was synthesized and applied for the co-delivery of Akt1 shRNA and paclitaxel. Biodegradable poly(β-amino ester) (PAE) composed of low molecular weight polyethylenimine and poly(ethylene glycol) was used as a carrier for Akt1 shRNA. The PAE exhibited high transfection efficiency and low toxicity in MCF-7 and MDA-MB-231 breast cancer cell lines. Akt1 shRNA complexed with PAE exhibited significantly gene silencing efficiency in MCF-7 breast cancer cells. Moreover, reduction of Akt1 expression increased the in vitro chemosensitivity of MCF-7 cells with paclitaxel and increased paclitaxel-induced apoptosis. The shRNA-induced knock-down of Akt could be a novel and potent strategy to sensitize cancer cells to paclitaxel for more efficient chemotherapy. |
| 저자 |
곽정정, Rohidas Arote, Tao Jiang, 강호림, 조명행, 조종수
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| 소속 |
서울대 |
| 키워드 |
Akt; paclitaxel; shRNA; apoptosis; poloxamer; hydrogel
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| E-Mail |
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