| 학회 |
한국공업화학회 |
| 학술대회 |
2006년 가을 (11/10 ~ 11/11, 경희대학교(수원캠퍼스)) |
| 권호 |
10권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Capillary Electrophoresis-Single Strand Conformation Polymorphism for monitering bacteria population |
| 초록 |
The single-strand conformation polymorphism(SSCP) is a DNA or RNA separation method based on changes in the secondary structure caused by a change in sequence. This study was performed to developed the identification technique of different bacteria in microbial community using capillary electrophoresis single-strand conformation polymorphism of four different specific genes were specified region genes, 16S rRNA gene, 23S rRNA gene, GyrB gene, and EF-Tu gene. The specific PCR primers were designed. For SSCP analysis, the PCR products were denatured , injected and separated by Capillary electrophoresis. Data showed that the peak in the data was correlated with the number of bacteria. |
| 저자 |
김진1, 정주희2, 남명희2, 정규열3, 오민규1
|
| 소속 |
1Korea Univ., 2Korea Basic Science Institute, 3Pohang Univ. of Science and Technology |
| 키워드 |
Capillary Electrophoresis; SSCP; GyrB gene
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| E-Mail |
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