| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.1257 |
| 발표분야 |
분자인식기반 고도분리기술 심포지엄 |
| 제목 |
Multiplexed, high-throughput SNP analysis by free-solution conjugate microchannel electrophoresis |
| 초록 |
Technologies that allow for high-throughput, economical, and accurate single nucleotide polymorphism (SNP) genotyping are becoming crucial for modern genomic analyses. Here, we present a method for multiplexed single-base extension (SBE) genotyping that takes advantage of the unique separation modalities made possible via end-labeled free-solution electrophoresis (ELFSE). A series of monodisperse "drag-tags" was created using both chemical and biological synthesis and used to achieve the high-resolution separation of genotyping reaction products by microchannel electrophoresis without a polymeric sieving matrix. A highly multiplexed SBE reaction was performed in which 16 unique drag-tagged primers simultaneously probe 16 p53 gene loci, with an abbreviated thermal cycling protocol of only 9 minutes. The drag-tagged SBE products were rapidly separated by ELFSE in both capillaries and microfluidic chips with genotyping accuracy in excess of 96%. The separation requires less than 70 seconds in a glass microfluidic chip, or about 20 minutes in a commercial capillary array sequencing instrument. |
| 저자 |
원종인1, Annelise E. Barron2
|
| 소속 |
1홍익대, 2Northwestern Univ. |
| 키워드 |
SNP; SBE; electrophoresis
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
